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By J. H. Jones
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Additional info for Amino Acids, Peptides and Proteins (SPR Amino Acids, Peptides, and Proteins (RSC)) (Vol 1)
A. Vandenheuvel, Analyt. , 1968,25, 452. S. Shaltiel, Biorhem. Biophys. Res. , 1967, 29, 178. G . L. Cailewaert and C. A. Vernon, Biochem. J . , 1968, 107, 728. W. R. Gray, Methods in Enzymology, 1967, 11, 139. C. Gros and B. Labouesse, European J . , 1969, 7 , 463. D. Morse and B. L. Horecker, Analyt. , 1966, 14, 429. C. Gros, Bull. chim. France, 1967, 3952. 40 Amino-acids, Peptides, and Proteins DNS-amino-acids on thin-layer silica gel have been described, but Gray 67 warns that in his experiments with that technique there was a rapid destruction of dansyl amino-acids adsorbed on to silica.
G. Scheme 8) because hydrogen on the H O . CO,I Me ---- H,O + H,&CH-CO~I *CH2 Scheme 8 a-carbon atom is deactivated towards the electrophilic hydroxyl radical by the protonated amino-group. Some amino-acids also suffered hydrogen abstraction from other positions in the side-chain, but only glycine (which has little alternative) gave a radical formed by loss of a hydrogen radical from the a-carbon atom. Amino-acid analysis of the ninhydrin-positive products of these reactions showed that the products were identical with those produced by y-irradiation, confirming the validity of the model.
Mol. , 1968, 34, 379. ‘Methods in Enzymology,’ ed. S . P. Colowick and N. 0. Kaplan; vol. 11, ed. C. H. W. Hirs, Academic Press, New York, 1967. J. , Elsevier, Amsterdam and London, 1967. Structural Investigation of Peptides and Proteins 33 amino-acid analysis. Methods, both chemical and enzymic, for achieving the hydrolysis of a protein into its constituent amino-acids have been covered in a recent review and need not be repeated here. ' Many methods are available for the analysis of amino-acid mixtures and these will be considered in turn.
Amino Acids, Peptides and Proteins (SPR Amino Acids, Peptides, and Proteins (RSC)) (Vol 1) by J. H. Jones