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By Hartmut Franz (auth.), Prof. Dr. Dr. Hartmut Franz, Ken-ichi Kasai, Jan Kocourek, Sjur Olsnes, Leland M. Shannon (eds.)

ISBN-10: 3333005840

ISBN-13: 9783333005843

ISBN-10: 3662267519

ISBN-13: 9783662267516

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Extra resources for Advances in Lectin Research: Volume 4

Sample text

CPBC and CRP (POD labeled) compete for phosphoryl choline bound to bovine serum albumin (BSA) on the solid phase "' 10 d /';. NaCI/1ml/mouse 0 150ng ML I/1ml/mouse e 300ng ML I/1ml/mouse A 1000ng ML I/1ml/mouse 2 3 4 5 6 10 d Fig. 4 Estimation of mouse CPBC after i. p. injection of ML I. After application of 150ng a rapid increase followed by a decrease after day 2 is seen. 300ng effect a two (or three) peak curve. Mter application of 1000 ng/mouse the increase continues till the death of the animal at day 2.

The corresponding activity of the A chain which is not a galactose specific lectin but a Nglycosidase indicates that the mediator-releasing effect of ML I is not necessarily due to its galactose binding activity. Ziska et al. (1985) described a procedure for the preparation of mistletoe extracts with a defined content of lectins for use in therapy, mainly in cancer treatment. Using ultrafiltration with different membranes a lectin-free fraction was received. By addition of concentrated fresh extracts of Viscum album with a determined lectin content the lectin concentration of the final solution was adjusted.

1989) compared in detail the sugar specificity of ML I with that of ML II and ML III, respectively. ML II and ML III have been purified using FPLC ion exchange chromatography on a Mono S cation exchanger. 5). 02 M). Among the inhibiting D-galactosides, differing only in the configuration at the anomeric C-1 atom, there seems to be a slight preference for ~-anomers. Aromatic aglycons of D-galactosides increased binding of the corresponding sugars to alllectins. 5 200 200 200 12 12 3 3 12 12 200 200 12 6 6 3 6 3 25 25 200 25 25 3 3 12 12 200 200 50 3 12 6 6 3 25 25 200 25 25 3 3 12 12 200 200 Methyl-~-D-galactopyranoside Phenyl-a-D-galactopyranoside Phenyl-~- D-galactopyranoside 2-Deoxy-D-galactose 2-0-Methyl-D-galactose 3-0-Methyl-D-galactose 6-0-Methyl-D-galactose 6-Deoxy-D-methyl-galactose Lactose (~-1-4) Lactulose (~-1-4) Melibiose (a-1-6) Raffinose (a-1-6, ~-1-2) D-Talose (C-2 epimer) D-Gulose (C-3 epimer) topyranosyl ring system abolished the binding to the lectin.

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Advances in Lectin Research: Volume 4 by Hartmut Franz (auth.), Prof. Dr. Dr. Hartmut Franz, Ken-ichi Kasai, Jan Kocourek, Sjur Olsnes, Leland M. Shannon (eds.)


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